![]() Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, Proteus mirabilis ATCC 35659, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 27853 were used as quality-control strains. Interpretation of the results was based on European Committee on Antimicrobial Susceptibility Testing (EUCAST) breakpoints ( ) for Enterobacterales. Īntimicrobial susceptibility testing (AST) was performed for ampicillin, amoxicillin–clavulanic acid, chloramphenicol, ciprofloxacin, ceftriaxone, doxycycline, gentamicin, amikacin, meropenem, piperacillin-tazobactam, and sulfamethoxazole–trimethoprim, using E-test (Liofilchem, Abruzzo, Italy) on Mueller–Hinton agar plates. The methodology of sample preparation for MALDI-TOF MS measurements has been described elsewhere. Between 20, presumptive phenotypic (biochemical reaction-based) methods and VITEK 2 ID (bioMérieux, Marcy-l’Étoile) were used for bacterial identification, while after 2013, this was complemented by MALDI-TOF MS (Bruker Daltonik Gmbh, Germany). All culture media were incubated at 37 ☌ for 24–48 hr in a 5% CO 2 atmosphere, 5% sheep-blood agar, chocolate agar, and eosin methylene blue agar. Blood-culture bottles were incubated for 5 days (21 days, if endocarditis was suspected) in the aforementioned detection systems. ![]() Between 20, the BD Bactec (Beckton Dickinson, Franklin Lakes, NJ, USA) detection system was employed in our Institute for the incubation of blood-culture bottles, while from 2013 onward, the BacT/ALERT 3D (bioMérieux, Marcy-l’Étoile, France) detection system was used. Sample processing in our Institute was carried out according to guidelines for routine clinical bacteriology, which have been previously described. Data on affected patients were also collected, limited to demographic characteristics (age, sex, and inpatient/outpatient status), indication for sample submission, and the administered empiric antibiotic therapy. Only the first isolate per patient was included in the study however, isolates with different antibiotic-susceptibility patterns were considered as different individual isolates. Data were collected for samples positive (10 5 or more CFU) for Pantoea species using the Institute’s Laboratory Information System. The Clinical Center has a bed capacity of 1,820 beds and annually serves more than 400,000 patients in the region, according to the data of the Hungarian National Health Insurance Fund (NEAK). The present retrospective study was based on microbiological data we collected between Januand Decemat the Institute of Clinical Microbiology (University of Szeged), the principal clinical microbiology laboratory of the Albert Szent-Györgyi Clinical Center in Szeged, Hungary. ![]() The aim of this study was to describe the prevalence and antibiotic susceptibility patterns of Pantoea species isolated at a tertiary-care teaching hospital in Hungary during a 12-year period (2006–2017). The spectrum of verified (i.e., clinically significant) bacterial pathogens has significantly broadened in recent years, due to the introduction of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and 16S rRNA sequencing in the routine diagnostic workflow of microbiology laboratories. ![]() ![]() The epidemiology and resistance trends of bacterial pathogens vary greatly by the profile of the healthcare institution and geographical region therefore, the assessment of local data is essential to evaluate trends over time and to reflect on international data. agglomerans bacteremia with antacid/proton pump inhibitor use (corresponding to a decreased protective effect of gastric acid) and gastrointestinal mucosal injury have been described, which may be attributable to the abundance of P. Most of the affected patients in the literature had severe underlying illnesses (hematological malignancies or solid tumours, manifest AIDS) however, infections associated with penetrative trauma, contaminated intravascular catheters and fluids (dialysis and intravenous anesthesia), and agricultural occupation have also been reported. It is generally considered a rare, opportunistic pathogen with low virulence, mainly implicated in nosocomial outbreaks affecting neonates and immunocompromised patients. Pantoea agglomerans (previously known as Erwinia herbicola and Enterobacter agglomerans ) is the most frequently isolated member of the genus in the context of human infections. Pantoea species are pigmented, facultative anaerobic Gram-negative rods belonging to the Enterobacterales order and frequently isolated from natural environments (e.g., plants, soil, and water). ![]()
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